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Products / Microscopes /Confocal Microscope /Laser Point Scanning Confocal Microscope /

Laser Point Scanning Confocal Microscope

Overview Specifications Upgrade Options Applications Software Detector Selection Order Now!

SIMSCOP Laser Point Scanning Confocal Microscope — Nano Series Product Overview

SIMSCOP Laser Point Scanning Confocal Microscope — Nano Series is engineered specifically for advanced scientific research and precision industrial inspection. The platform delivers major breakthroughs in operational flexibility and collection efficiency: natively supporting up to 4-channel simultaneous imaging with acquisition frame rates reaching 16 fps@512×512 and 32 fps@256×256, offering dependable performance for dynamic life science research. A versatile selection of detection schemes enables users to optimize signal-to-noise ratio according to experimental needs. Featuring an architecturally reserved, highly modular design, the system supports seamless upgrades to Raman/fluorescence spectroscopy, NIR I/II imaging, and multiphoton microscopy. Combined with intuitive automated control software that significantly lowers the threshold for complex operations, it stands as a core microscopy platform with extensive upgradability and universal application value.

The Nano Series offers three dedicated configurations to meet diverse research requirements:

• Nano F HS: Inverted 4-Channel Confocal — Multi-channel simultaneous detection for peak imaging throughput.

• Nano D: Inverted 2-Channel Confocal — Classic dual-channel setup balancing robust performance with routine imaging.

• Nano S: Upright Single-Channel Confocal — Upright architecture built for specialty material inspection and thick slice imaging.

SIMSCOP Laser Point Scanning Confocal Microscope Nano Series Appearance

Key Specifications

Lateral Resolution 230 nm @ 100x Oil objective

Imaging Depth < 100 μm

Laser Wavelengths 405 nm / 488 nm / 561 nm / 640 nm

Detection Range 400 – 700 nm

Scanning Speed Up to 16 fps@512×512; 32 fps@256×256

Field of View (FOV) Φ18 mm inscribed square

Detection Efficiency Peak Photon Detection Efficiency (PDE) up to 41%

Key Advantages

✔ Simultaneous Multi-channel: Up to 4 channels imaged simultaneously, preventing spectral crosstalk and scan latency.
✔ High-Speed Acquisition: High frame rate scanning (up to 16 fps@512×512) captures live-cell microscopic dynamics.
✔ Flexible Configurations: Laser lines and multi-grade detector types can be freely customized and combined.
✔ Automated Control: Unified automation software simplifies multi-dimensional acquisition workflows.

Widefield vs. Confocal Imaging Comparison

Fig: Widefield vs. Confocal Imaging Comparison (Mouse Brain Neurons 40X)

Simultaneous Multi-channel Imaging: Supporting up to 4 detector channels operating concurrently, the platform acquires pure signals from distinct fluorophores or molecular probes across emission spectra. This eliminates phototoxicity and fluorophore bleaching associated with sequential scanning, making it suitable for thick biological specimens and multi-target molecular interaction studies.

System Optical Layout

SIMSCOP Laser Point Scanning Confocal Microscope Optical Diagram

Parameter Module		Nano D	Nano F HS	Nano S
Laser Module	Laser Type	Standard Wavelengths: 405±5 nm; 488nm±5nm; 561nm±1nm; 640nm±5nm Output Mode: Single-mode Polarization-maintaining Fiber Coupled (TEM00) Single-wavelength Output Power: ≥ 20mW Power Stability: < 1% Spectral Linewidth: < 3nm TTL Modulation: 1kHz Laser Power Adjustment Accuracy: 0.1%, Multi-wavelength AOTF Power Adjustment Optional Wavelengths: 375/445/473/515/525/532/633/660/685/785/808nm Optional Modulation: AOTF/AOM, ≥ 30mW per line; Optional Power: Reserved high-power version (up to 50mW class)		Standard Wavelength: 405nm Output Power: ≥ 20mW Power Stability: < 1% Power Adjustment Accuracy: 0.1% Spectral Linewidth: < 0.1nm
Confocal Module	Scan Head Design	Modular scan head (detachable for maintenance); Integrated scanning/detection orientation design (higher spatial light detection efficiency)
	Scanning Mode	Arbitrary combination of multi-dimensional scanning modes: Point scan, Rotation, X-Y-Z-t-λ, enabling 5D image capture and display. Multi-channel fluorescence imaging, time-series scan, multi-position acquisition, Z-stacking, and panoramic stitching Additional spectral detection port		Arbitrary combination of multi-dimensional scanning modes: Point scan, Rotation, X-Y-Z-t, enabling 4D image capture and display. Time-series scan, multi-position acquisition, Z-stacking, and panoramic stitching Suitable for automated inspection of large-sized samples (such as wafers, PCB boards).
	Max Scanning Resolution	≥4096×4096 standard; Hardware/transmission reserved for 8192×8192
	Scanning Speed (Typical)	8 fps@512x512; 16 fps@256x256	16 fps@512x512; 32 fps@256x256	4 fps@512x512; 8 fps@256x256
	Field of View (FOV)	Φ18mm inscribed square
	Lateral Resolution	230nm @ 100x Oil objective
	Imaging Depth	< 100um
	Pinhole & Bit Depth	Fixed pinhole (optional sizes: 10-100um); Motorized pinhole option: Φ0 - Φ11.5 mm, minimum motion increment: 0.01mm Image Bit Depth: 16 bit
Detector Module	Detection Configuration / Method	Side-mounted 2-Channel Confocal Dual Detectors: UV-Vis Detector + Red Detector (High-end optional)	Side-mounted 4-Channel Confocal Four Detectors: UV-Vis Detector + Blue-Green Detector + 2× Red Detectors (High-end optional)	Upright Single-Channel Confocal Single Detector: UV-Vis Detector
	Detector Channel Specifications	UV-Vis Detector: Peak wavelength 420nm, Response spectrum 300~950nm, Photon detection efficiency 41% Red Standard Detector: Peak wavelength 630nm, Spectral range 300~920nm, Cathode quantum efficiency 15% High-end Option (High-sensitivity Red): Peak wavelength 660nm, Spectral range 300~840nm, Cathode quantum efficiency 25%	UV-Vis Detector: Peak wavelength 420nm, Response spectrum 300~950nm, Photon detection efficiency 41% Standard Options: • Blue-Green Standard Detector: Peak 420nm, Range 300~650nm, Cathode quantum efficiency 24% • Red Standard Detector: Peak 630nm, Range 300~920nm, Cathode quantum efficiency 15% High-end Options: • Blue-Green High-Sensitivity Detector: Peak 520nm, Range 300~740nm, Cathode quantum efficiency 45% • Red High-Sensitivity Detector: Peak 660nm, Range 300~840nm, Cathode quantum efficiency 25%	UV-Vis Detector: Peak Wavelength: 420nm Response Spectrum: 300~950nm Photon Detection Efficiency: 41%
	Detection Bands & Filters	400-700nm; 4-channel dichroic mirror: 405/488/561/640 6-position motorized filter wheel; Filter: 452nm/45, 530nm/43, 607nm/36, 685nm/40*, Blocking efficiency OD≥6	400-700nm; 4-channel dichroic mirror: 405/488/561/640 Filter: 452nm/45, 530nm/43, 607nm/36, 685nm/40*, Blocking efficiency OD≥6	405nm
Microscope Module	Matching Microscope Platform	Fully Motorized Fluorescence Microscope		Self-developed Upright Microscope Frame
	Eyepiece	30° inclined trinocular tube (100:0, 20:80, 0:100), wide field of view (FN23) eyepiece 10X/23mm, diopter adjustable		/
	Transmitted Koehler Illumination	Koehler illumination 100W long-life halogen lamp		/
	Epi-illumination	LUMOS high-brightness LED fluorescence light (optional mercury lamp or metal halide lamp)		12V/100W long-life halogen bulb
	Motorized Long Working Distance Condenser	Abbe condenser N.A. 0.90 (optional turret phase contrast / darkfield condenser, LWD N.A. 0.65 WD 10.7 condenser, swing-in/swing-out condenser)		/
	Nosepiece & DIC Slider	Encoded / Motorized 6-hole nosepiece 4X phase contrast ring plate set (0.55, Ph0); 10X/20X/40X phase contrast ring plate set (0.55, PH1)		5-hole nosepiece (DIC Slider: /)
Stage Module	XY Stage (High-precision Motorized Stage)	Stepper motor XY microscope stage Travel range: 120mm(X) × 80mm(Y); Max speed: 30mm/s; Resolution: < 1um; Uni-directional repeatability: ≤ 1um; Repeatability: ≤ 2.5um; Horizontal load: 1kg		Stepper motor XY microscope stage Travel range: 25mm × 25mm; Max speed: 6mm/s; Resolution: 0.00025 mm/pulse; Repeatability: ±2um; Horizontal load: 4kg
Stage Module	Focusing System (Z-axis Control)	Travel range: 500μm; Closed-loop resolution: 30nm; Repeatability: 0.5um; Load capacity: 200g		Travel range: 12mm; Resolution (20 microsteps): 0.125um; Repeatability: 5um; Load capacity: 20kg

Upgrade to Confocal Raman Microscope ● 532,785,1064 Raman ● Upright Microscope setup ● High Resolution with Raman image mapping	Details Click here

Upgrade to Fluorescence lifetime imaging microscopy (FLIM) FLIM is a type of microscopy that allows for the visualization and analysis of biological samples based on the fluorescence lifetime of the fluorophore being used. FLIM measures the time between the excitation and emission of photons in a sample, which can provide information about the properties of the fluorophore and the environment in which it is located. FLIM can be used to study a wide range of biological processes, including protein-protein interactions, enzyme activity, and ion concentration changes. It is often used in combination with other imaging techniques, such as confocal microscopy, to provide more detailed information about the sample.

Upgrade to Single / Two /Multi Photon Microscope In two-photon microscope, a laser emits light at a specific wavelength that is absorbed by the fluorescent molecules in the sample. When two photons of this light are absorbed simultaneously, they provide enough energy to excite the fluorescent molecule and cause it to emit light at a longer wavelength, which can be detected by the microscope. Because two photons are required to excite the molecule, the probability of fluorescence emission is low and only occurs at the focal point of the microscope, allowing for high-resolution imaging and greater depth than conventional microscopes. Two-photon microscopy has a number of applications in neuroscience, biology, and biomedical imaging. For example, it has been used to study the activity of individual neurons in the brain, visualize the structure and function of blood vessels, and track the behavior of cells in living tissues.

Upgrade to Confocal Spectral Microscope (Near IR I/II Confocal) ● Upgrade to Confocal Spectral Microscope (NIR I/II confocal) ● Wavelength Range UV to NIR (200nm-2.5nm) ● Spectral resolution up to 0.2nm ● Large NA setup for high-sensitivity application	Details Click here

Upgrade to High Speed Lines Scan Confocal Microscope ● Frame Rate 210fps ● Resolution: 150 nm over the optical diffraction limit ● Imaging Depth of 500 to 1000 microns ● Image Contract enhancement 20-30 dB	Click Here for More Info

Upgrade to Terahertz Confocal Microscope System ● 100GHz, output power: 80mW ● Spatial resolution 150-200um The terahertz confocal microscope uses a focused beam of terahertz radiation to scan the sample being analyzed. This beam is then reflected back and collected by a detector, which creates an image of the sample based on the intensity of the reflected radiation. By using a confocal design, this microscope can achieve high resolution and can selectively focus on different depths within a sample. it can be used to study the microstructure and properties of materials, such as polymers, ceramics, and semiconductors, and to detect defects or anomalies in their structures. In biology and medicine, it can be used to image and study biological tissues, including skin, teeth, and cartilage, which are transparent to terahertz radiation.

Upgrade to Super Resolution Confocal Re-scan Structure illumination Microscope A "re-scan" confocal microscope is a type of confocal microscope that uses a rapidly moving mirror or scanner to scan the laser beam across the sample multiple times, producing even higher resolution and better contrast images than standard confocal microscopes. Overall, re-scan confocal microscopes are very powerful tools for studying biological tissues, cells, and other samples, and are widely used in research labs, medical facilities, and other scientific settings

Upgrade to Low Temperature Confocal Microscope Compatible with SIMTRUM Cryostat to perform Low-temperature Raman measurements -190 to 600 degrees ● 8 probe arm able to upgrade to adjustable probe arm ● Reflection or transmission mode available

SIMSCOP Single-Point Confocal Microscope Real Acquisition Gallery —— Application Cases

BPAE cells stained with MitoTracker™ Red CMXRos, Alexa Fluor™ 488 Phalloidin, and DAPI under a 60X water immersion objective (NA 1.2)

BPAE cells#1–60X–405nm

BPAE cells#1–60X–488nm

BPAE cells#1–60X–561nm

BPAE cells#1–60X–Composite

BPAE cells stained with mouse anti-α-tubulin, BODIPY™ FL goat anti-mouse IgG, Texas Red™-X Phalloidin, and DAPI under a 60X water immersion objective (NA 1.2)

BPAE cells#2–60X–405nm

BPAE cells#2–60X–488nm

BPAE cells#2–60X–561nm

BPAE cells#2–60X–Composite

Microtubules

Nucleus

Merge

The image above shows a confocal microscopy image of cultured human gingival fibroblasts. Interphase microtubules (green) were labeled with an anti-α/β-tubulin primary antibody. A FITC-conjugated secondary antibody was then applied. Nuclear DNA (blue) was stained with Hoechst 33242.

Cycad leaf. 20X

Spinach root stem. 20X

Wheat seed. 20X

Mouse nerve single-point scanning image, 20x objective,
Detector: SiPM (This image has not undergone post-processing)

Fibrous connective tissue section, 10X objective_1000x1000,
Detector: SiPM (This image has not undergone post-processing)

Live mitotic Hela cells treated with epsin1 siRNA and DiOC6(3) to label mitotic membranes (green). Confocal images were taken along the Z-axis with a step size of 0.118μm.

Pollen grain - 3D

SIMSCOP Laser Scanning Confocal Microscope——Nano Series Software Overview

GUI Control Panel

Software Features

Domestically developed software with one-click switching between Chinese and English interfaces.
Intelligent configuration: supports sequential or simultaneous scanning for 4 channels, as well as sequential or simultaneous scanning for single/multiple channels based on dye specifications and diverse application requirements.
Native software integrates X, Y, Z, λ, and T 5D scanning into a unified platform for acquisition, processing, and visualization without relying on third-party software.
Real-time multicolor scanning and real-time multicolor image overlay, supporting multidimensional (X, Y, Z, T) confocal image acquisition, processing, and reconstruction.
The acquisition software includes built-in 3D visual reconstruction, arbitrary spatial sectioning, and interactive volumetric rendering. Image acquisition and 3D visualization are fully integrated into one single application.
Region measurement feature: allows drawing arbitrary ROIs on images to measure quantitative metrics such as area and length.
Reassembles acquired Z-stack image sequences, allowing users to interactively browse the sample in 3D.
Built-in motorized microscope component control, including nosepiece rotation, fluorescence filter turret rotation, condenser turret rotation, motorized stage navigation, and Z-axis focus tracking, supporting automated slide scanning.
Equipped with high-performance large-image tiling and stitching, achieving automated laser confocal scanning and immediate review of stitched mosaics.
Intelligent optical path routing: offers two optical configuration modes (widefield fluorescence and confocal), configuring all optical paths with a single click.
Integrated 3D large-area stitching: seamlessly executes automated laser confocal wide-area image stitching simultaneously with 3D Z-Stacks acquisition.
Deconvolution image processing capabilities (optimized primarily for widefield, with support for point scanning).
FRAP (Fluorescence Recovery After Photobleaching) analysis workflow with post-photobleaching recovery curve data export.
Scale calibration, fluorescence intensity quantification, denoising filters, optical filtering algorithms, and comprehensive data logging.
Automated cell identification and binary image segmentation.

SIMSCOP Fluorescence Spot Scanning Confocal Microscope——Detector Selection and Performance Analysis

Flexible Detector Type Selection (GaAsP, PMT/MA, PMT/SiPM): The ability to select detector types allows you to optimize SNR according to your specific experimental requirements [cite: 8]. The combination of multiple detectors and functional information enables quantitative analysis of fluorescence intensity, emission spectra, and fluorescence lifetime measurements [cite: 8]. This is crucial for many quantitative biology and materials science experiments [cite: 8].

Parameter	SiPM	GaAsP PMT	Multialkali PMT	Ultra Bialkali PMT
Photosensitive Area	6mm × 6mm	Φ5 mm	Φ8 mm	Φ8 mm
Spectral Response Range (nm)	200-900	300-740	185-870	230-700
Peak Response Wavelength (nm)	420	520	400	400
Dark Current nA	900	3	1	1
Peak Wavelength Detection Efficiency	38% @ 420nm	45% @ 520nm	23.87% @ 400nm	40.3% @ 400nm
Rise Time	180ps	1ns	0.57ns	0.57ns

Detector Performance Curves

UV-Vis Detector

Red Standard Detector

Red High-Sensitivity Detector

Blue-Green Standard Detector

Blue-Green High-Sensitivity Detector

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